ABOUT THE GENOMICS FACILITY AT CCHMC
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The facility is a technical and expertise resource for the genomic research community at Children's Hospital and the University of Cincinnati.
We offer a wide variety of services for DNA Sequencing and Genotyping.
We are located in room 1045A of the Research Foundation Building. Our regular hours of operation are 9:00 am to 5:00 pm on weekdays. We use the ABI PRISM® 3730 DNA Analyzer, a capillary electrophoresis system developed specifically for production-scale DNA analysis. Sequencing accuracy is > 98.5% to 550 base pairs. Read length can reach over 800 bp with good starting materials. The current capacity is around 1080 sequence reads, or over 10,000 microsatellite based genotypes per day, 7 days a week. |
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The facility is also equipped with two TECAN robotic laboratory workstations to automate sequencing reaction setup, dye terminator removal, plasmid minipreps and purifications.
We can currently produce 384 high quality plasmid minipreps overnight, 4 nights week from bacterial pellets. During the day we can reach an average throughput of one miniprep per minute.
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CONTACT US
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Laboratory (513) 636-2801 dnacore@cchmc.org David Fletcher (513) 636-2801 David.Fletcher@cchmc.org |
Mehdi Keddache (513) 636-0122 Mehdi.Keddache@cchmc.org Katie Lutz (513) 636-2801 claa3x@cchmc.org |